Protein Gel Electrophoresis: Retaining Protein Function with Native Gel

Which kind of gel retains protein function?

Final answer: A) Native gel


The type of gel that retains protein function is B) Native gel. Native PAGE allows for the separation of proteins based on their charge, molecular weight, and shape, without denaturing them. This means the proteins maintain their native conformation and, therefore, their function. In contrast, denaturing gels, such as those used in SDS-PAGE, add a detergent such as SDS to unfold proteins into linear molecules, which then solely separates proteins based on size. Reducing gels break disulfide bonds, denaturing proteins further, and stacking gels are part of the SDS-PAGE apparatus that helps concentrate the proteins before separation but do not maintain their native function.

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